Lemna aequinoctialis is one of the most widespread duckweed species in tropical and subtropical regions, with a range spanning the Americas, Africa, Asia, and the Pacific. In Florida, it is frequently found in warm, slow-moving or still freshwater: ponds, ditches, roadside swales, marshes, and lake margins.
The plant is among the smallest flowering plants on Earth. Each frond is 1 to 3 millimeters wide, oval to elliptical, slightly asymmetrical, and bears a single trailing rootlet. New fronds bud directly from the parent frond through vegetative reproduction; a single frond can produce a doubling of biomass in as little as one to two days under ideal conditions of warm temperature, high light, and sufficient dissolved nitrogen.
Growth rate is highly sensitive to dissolved nitrogen availability. Like all Lemna species, L. aequinoctialis requires a continuous supply of inorganic nitrogen for rapid budding. In nutrient-poor or cool conditions, growth slows dramatically and introduced biomass is consumed before expansion occurs. Its thermal optimum is approximately 25 to 32 degrees Celsius, making it better adapted to warm subtropical and tropical systems than the more temperate Lemna minor.